The Effects of Nitric Oxide on Some Early Germination Parameters and Mitotic Activity In Lentil

[摘要] Germinationofseedsisacrucialstageinplantlifecycle.ItisknownthatNOalsoplaysanimportantroleinseeddormancyandgermination.LentilisoneofthemostimportantcropsinTurkey.Hencewechosethisplantasanexperimentalmaterial.TheaimofthestudyistoexamineeffectsoffourdifferentconcentrationsofSNP(0,01μM,100μM,600μMand700μM),aNOdonor,onlentilseeds.Weestablishedgerminationrate,primaryrootlength,proteinamount,activitiesofenzymessuchasamylase,acidphosphatase,proteaseaswellasmitoticindexat0,24,48and72hours.Wefoundthat100μMSNPinducesgerminationoflentilseeds,whereas700μMSNPclearlyinhibitsit.Itwasalsoestablishedthat100μMSNPwashighlyeffectiveinprimaryrootlengthandacidproteaseactivity.TotalsolubleproteinamountswerefoundtobehigherthanthecontrolgroupinallSNPapplicationsdependingontheconcentrations,andthemostobviousincreasewasseenintheapplicationof700μMSNPwhichalsoenhancedamylaseactivity.AllSNPapplicationshadthesameeffectonacidphosphataseactivity,whereasacidandneutralproteaseactivitieschangeddependingontimeandconcentration.Inaddition,itwasestablishedthat700μMSNPcausedareductioninmitoticactivity.Inthelightofthefindingsobtainedinthisstudy,itcanbeclaimedthatdependingontheconcentration,Asaresult,NOhasanoteworthyeffectasaplantgrowthregulatoronthegerminationoflentilseeds.

[学科分类] 生物科学（综合）

[关键词] Nitric oxide;germination;protein;germination enzymes;mitotic index