Carbon and Nitrogen Sources for Lipase Production of Micrococcus sp. Isolated from Palm Oil Mill Effluent-Contaminated Soil

[摘要] This research aim to optimize the lipase production of Micrococcus sp. isolated from oil palm contaminated soil. The production of lipase enzymes is carried out in modified medium containing olive oil, sea salt, yeast extract with various types of carbon source (glucose, sucrose, glycerol) and nitrogen sources (tryptone, ammonium phosphate, urea and ammonium nitrate). The activity of lipase enzyme was determined by UV-Vis spectrophotometry method toward ρ-nitrophenylpalmitate (ρ-NPP) as a substrate. The results showed that glucose 1% was better carbon source compared with sucrose and glycerol. Ammonium phosphate, urea and ammonium nitrate were good nitrogen sources for lipase production of Micrococcus sp., although tryptone was the best nitrogen source.

[发布机构] Departement of Chemistry, Faculty of Sciences and Technology, Universitas Airlangga, Kampus C Unair Jl. Mulyorejo, Surabaya; 60115, Indonesia^1

[效力级别] 化学 [学科分类] 生物科学（综合）

[关键词] Ammonium phosphates;Carbon and nitrogen;Carbon source;Contaminated soils;Micrococcus sp;Nitrogen sources;Palm oil mill effluents;UV-vis spectrophotometry