Aspects of nucleic acid metabolism during development of the brine shrimpArtemia salina
[摘要] Encystedembryosofthebrineshrimp,Artemiasalina,containatleastfivespeciesofRNAwhichareresolvablebychromatographyoncolumnsofmethylatedalbuminofKieselguhr(MAK).Duringdevelopmenttheproportionofeachspecieswithintheembryoremainsrelativelyconstant.Quantitativeextractiondataalongwith32Piandadenosine-3HincorporationstudiesindicatethatRNAsynthesisduringdevelopmentofArtemiaismostintenseimmediatelyafterhatching,whereasverylittleRNAsynthesis,ifany,occursbeyond36hoursdevelopmentat30°C.DNAsynthesisbeginsshortlyafterhatchinganditcompleteby60hours.DuringtheperiodofintenseRNAsynthesis(20–36hours),certaintRNAspeciesandheavyRNA(hRNA)appeartobethemostmetabolicallyactiveRNAfractions.TheexactfunctionofhRNAduringdevelopmentofArtemiasalinaremainstobeelucidated.
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[效力级别] [学科分类] 生物科学（综合）
[关键词] DNA;deoxyribonucleic acid;RNA;ribonucleic acid;DEAE;diethylaminoethyl;MAK;methylated albumin on kieselguhr;EDTA;ethylenediaminetetraacetic acid;AMP;adenosine 5′-monophosphates;ADP;adenosine 5′-diphosphates;ATP;adenosine 5′-triphosphates;GMP;guanosine 5′-monophosphates;GDP;guanosine 5′-diphosphates;GTP;guanosine 5′-triphosphates;Gp4G or diguanosine tetraphosphate;P1;P4-di-guanosine 5′-tetraphosphate;UMP;uridine 5′-monophosphate;UDP-X;carbohydrate derivative of uridine 5′-diphosphate;Pi;orthophosphate [时效性]
