Protein synthesis in amphibian oocytes and early embryos☆
[摘要] KineticsofaminoacidincorporationhavebeenstudiedinoocytesofRanapipiensatallstagesfromthefull-grownovarianoocyte,throughtheperiodofpituitary-inducedmaturationandseveralhoursbeyondfertilizationorartificialactivation.Also,aminoacidpoolsizesinoocyteshavebeenmeasuredatallthesestages.Poolsizesaresubjecttoconsiderablenaturalvariationintheeggstakenfromdifferentanimals.However,therateofflowofaminoacidthroughthepooland,thus,therateofproteinsynthesis,arerelativelyconstantineggsatthesamestage,evenwhentakenfromdifferentanimals.Therateofproteinsynthesisisverylowintheovarianoocytefromanunstimulatedfemaleandinoocytesuptoabout20hoursafterpituitarystimulation.Atthistimetheraterisesrapidly,reachesamaximumlevelofabout0.5μgperoocyteperhourandremainsessentiallyconstantthereafter.Neitherfertilizationnorartificialactivationhaveanyobservablequantitativeeffectonproteinsynthesis.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
