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Growth and glycine incorporation in chick embryo explants☆
[摘要] 1.1.TheeffectsofseveralnaturalandsyntheticnutrientmediaweretestedontheDNAandprotein-glycinecontentsof11–13-somitechickembryosexplanted8or24hours.2.2.Markedincreasesinbothsubstanceswereobtainedwithexplantsonmediacontainingthenaturaleggnutrient.Explantsincludingmoreextensiveareasoftheextraembryonicmembranesshowedlargerincreasesonsuchmediathanexplantswithsmallerextraembryonicmembranes.3.3.Aglucose-containingmediumpromotedalargeincreaseinDNAandasmallincreaseinprotein-glycineonlyinexplantswithlargeextraembryonicmembranes.Othersyntheticmediahadnoeffectontheprotein-glycinecontentandpromotedonlyasmallincreaseinDNA.4.4.Underexplantationconditions,withrapidlyincreasingprotein-glycinecontents,incorporationofglycine-1-C11ledtoasmallincreaseinthespecificactivitiesoftheprotein-glycineorprotein-nitrogen.Underconditionsofslowgrowth,amuchgreaterincreaseinthespecificactivitieswasfoundtooccur.Thisisinterpretedtoindicateutilizationofdifferenttypesofprecursorsforproteinformationunderconditionsofrapidandslowgrowth.
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[效力级别]  [学科分类] 生物科学(综合)
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