Changes in the distribution of glutamic, lactic, and malic dehydrogenases in liver cell fractions during development of the chick embryo☆
[摘要] AlltheLDHactivityandmostoftheMDHactivityofchickliverhomogenatesoccurredinthesupernatantfractionduringallstagesofdevelopment.ChickliverwasfoundtodifferfromratliverinthatthemitochondriacontainednotightlyboundMDH.MitochondrialGDHactivityincreasedafterthetwelfthdayofincubation,andthisrisewasfollowedbyasuddendropofGDHactivityinthesupernatantat15daysofincubation.ThesupernatantGDHhasadifferentoptimumpHtothatofthemitochondrialGDH.EarlystagesofdevelopmentarecharacterizedbyahighproportionofsupernatantGDH.ThepossibilitythatthisphenomenonmightbeduetoatransferofGDHfromthesupernatanttomitochondriaisdiscussed.AlthoughtightlyboundGDHcouldsometimesbereleasedfrommitochondriabyultrasonicdisintegrationoraging,nosignificantdifferencesintheamountofGDHboundwithinthemitochondriaatdifferentstagesofdevelopmentwerefound.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
