Cytochrome oxidase deficiency during development of amphibian nucleocytoplasmic hybrids☆
[摘要] TheactivityofmitochondrialcytochromeoxidasewasinvestigatedduringtheembryonicdevelopmentofnucleocytoplasmichybridscontaininganucleargenomederivedfromR.pipiensandamitochondrialgenomederivedfromR.palustris.Usingaquantitativecytochemicalapproach,wefoundthattheactivityofcytochromeoxidasefailedtoincreaseduringthedevelopmentoftheseembryos.Controlembryoscontainingahaploidchromosomalcomplement,derivedfromthesamespeciesasthatfromwhichthematernallyinheritedmitochondriaisderivedandhybridcrossesbetweenR.palustrisandR.pipiens,showedasignificantincreaseincytochromeoxidaseactivityduringdevelopment.OxygenuptakedatafromdiploidandhaploidR.pipiensembryoswereinagreementwiththedataobtainedbythecytochemicalmethod.Theseresultsindicatethatanormalpatternofcytochromeoxidaseactivityduringembryonicdevelopmentrequiresanucleargenomewhichcontainsahaploidchromosomalcomplementderivedfromthesamespeciesasthatfromwhichthemitochondrialgenomeisderived.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
