Clusters of acetylcholine receptors located at identified nerve-muscle synapses in vitro☆
[摘要] Nerve-musclesynapsesformwithin24hrafterfragmentsofembryonicchickspinalcordareplacedinestablishedmusclecultures.Nerveprocess-musclefibercontactswerevisualizedwithinterferencecontrastoptics,andsitesoftransmitterreleasewereidentifiedbyextracellularstimulationofoverlyingneuritesinthepresenceoftetrodotoxin.Theresolutionofthisfocaldepolarizationtechniqueislessthan5μm,andresultsindicatethatsynapsesarerestrictedtoshortsegmentsalongunmyelinatedmotoraxons.Thedistributionofacetylcholine(ACh)receptorsoninnervatedmyotubeswasdeterminedbymicrointophoresisofACh.Clustersofreceptors,signaledbysharprelativepeaksofAChsensitivity,werelocatedexactlyatsitesoftransmitterrelease.Thedensityofreceptorswasnotincreasedallalongthelengthofmotoraxon-musclecontacts.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
