Prostaglandins and myoblast fusion☆
[摘要] PhysiologicalconcentrationsofprostaglandinE1(10−7and10−10M)provokeadiscreteburstofcellfusioninculturesofprimarychickmyoblasts,5hraftertheiradditionbutwellbeforethestartoffusion,undercontrolconditions.Twoinhibitorsofprostaglandinsynthesis,aspirin(2-acetoxybenzoicacid)andindomethacin(1-[p-chlorobenzoyl]-5-methoxy-2-methylindole-3-aceticacid),havebeenusedtoexaminethepossibilityofprostaglandinproductionbytheundifferentiatedmyoblasts.Bothinhibitorsproduceamarkedinhibitionofcellfusionwhichispossibletoreversebythefurtheradditionof10−5MprostaglandinE↑.ThefindingsprovideevidenceofprostaglandinsynthesisintheculturesandsuggestthatprostaglandinE↑isrequiredforthegenerationofatransientincreaseinintracellularcyclicAMPwhichbringsaboutthecellularchangesnecessaryforfusiontooccur.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
