Diversity of polyadenylated messenger RNA sequences in normal and 12-hr regenerating liver☆
[摘要] Molecularhybridizationtechniqueswereusedtoexaminethestructuralorganizationoftheratgenomeandgeneexpressionduringliverregeneration.ThenonrepetitivefractionofratDNAcomprisesapproximately69%ofthetotalandhasacomplexityof1.9×109nucleotidepairs.ComplementaryDNAs(cDNA)weresynthesizedusingpolyadenylatedpolysomalmRNAtemplatesfromnormaland12-hrregeneratinglivers.HybridizationofcDNAwithalargeexcessoftotalratDNAindicatesthattheproportionofpolyadenylatedpolysomalmRNAhomologoustorepetitiveandnonrepetitiveDNAisverysimilarinnormalandregeneratinglivers.HybridizationofcDNAswithexcesshomologousorheterologousmRNArevealedthat:(a)ThesequencecomplexityofpolyadenylatedpolysomalmRNAisessentiallythesameinnormaland12-hrregeneratingliver.Usingthesedataweestimatethatthemaximumproportionofsingle-copyDNArepresentedinpolyadenylatedpolysomalmRNAinliversofsham-operatedratsorin12-hrregeneratingliveris0.85–0.90%;(b)85–90%ofpolysomalpolyadenylatedmRNAiscommontonormalandregeneratinglivers,while11–14%isuniqueeithertonormalor12-hrregeneratingliverpolysomes.WecannotdiscernasyetwhetherthepolysomalpolyadenylatedmRNAsequencesuniquetonormalorregeneratingliversaretheresultofdifferentialgenetranscriptionand/orselectivetransportfromthenucleustopolysomes.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
