Differential activation of free and template-engaged RNA polymerase I and II during the resumption of development of dormant Artemia gastrulae
[摘要] RNApolymeraseactivitiesoftypeI(A)andII(B)canbedetectedinnuclearpreparationsfromdormantencystedgastrulaeofthebrineshrimpArtemiasalina,amajorpartofbothenzymesbeingfreeorfunctionallyunengagedtotheendogenoustemplate.Atthesametime,thedesiccatedgastrulaealreadycontainsmallamountsofbothRNApolymerasesinpreformedtranscriptionalcomplexes.Whenfurtherdevelopmentisinitiatedbyhydrationinaeratedseawater,largeincreasesinthefractionofenzymeastemplate-engagedpolymeraseIandIIactivitiesaccompanysmallerincreasesinthetotalandfreeenzymeactivities,sothatthetranscriptionallyactivecellshaveamuchlowerproportionofRNApolymeraseasfreeenzymethanhavethetranscriptionallyinactivedormantcells.AnalysisofthesechangesbyquantitationofthehydrolysisproductsoftheRNAsynthesizedinvitrobynucleiisolatedfromdormantgastrulaeandnaupliisuggeststhatanincreaseintheabsolutenumberoftranscribingpolymerasemoleculesoccursduringembryogenesis.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
