Changes in protein phosphorylation accompanying maturation of Xenopus laevis oocytes☆☆☆
[摘要] Proteinphosphorylationhasbeenmeasuredafterinjectionof[32P]phosphateintooocytesofXenopuslaevisundergoingprogesterone-inducedmeioticmaturation.Asoocytesmature,thereisaburstofnonyolkproteinphosphorylationseveralhoursafterprogesteroneexposureandshortlybeforegerminalvesiclebreakdown(GVBD).ThisburstisnotduetochangesinthespecificactivityofthephosphateorATPpool.Enucleatedoocytesexposedtoprogesteronealsoexperiencetheburst,indicatingthecytoplasmiclocationofphosphoproteinformation.Whenanoocytereceivesaninjectionofcytoplasmcontainingthematuration-promotingfactor(MPF),aburstofproteinphosphorylationoccursimmediately,andGVBDoccursshortlythereafter,eveninthepresenceofcycloheximide.Underavarietyofconditionspromotingorblockingmaturation,oocyteswhichundergoGVBDaretheonlyonestohaveexperiencedthephosphorylationburst.TheresultssuggestthattheproteinphosphorylationburstisanecessarystepinthemechanismbywhichMPFpromotesGVBD.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
