Factors involved in the stimulation of parasympathetic nerve outgrowth☆
[摘要] Nervegrowthfromthemouseparasympatheticsubmandibularganglionisstimulatedbythedevelopingtargetepithelium.Toinvestigatethenatureofthistrophicinfluence,homogenatesofsalivaryglands,gland-conditionedmedium,andformalin-fixedglandswereassayedforabilitytoelicitparasympatheticaxonextensionintissueculture.Neitherhomogenatesnorconditionedmediumstimulatedaxonoutgrowthfromsubmandibularganglia.However,whengangliawereaddedtoglandsinwhichproteinsynthesisandcellmovementhadbeenhaltedbyformalinfixation,stimulationofoutgrowthintothetissuewasobserved.Stimulationofaxonalgrowthoccurredafterhyaluronidaseandcollagenasetreatmentoftheglands,butnotaftertreatmentwithproteasesorwithheat.Moreover,prolongedformalinfixationdestroyedtheglandularabilitytoelicitaxonelongation.Intactgangliaculturedwithwholelivesubmandibularglandsinthepresenceoflowlevelsofhyaluronidaseorcollagenaseshowedextensiveaxonoutgrowthdespitedisruptionofthenormalmorphogeneticpatternofbothepitheliumandaxons.Ourresultssuggestthatstimulationofaxonoutgrowthdoesnotdirectlydependontheconcomitantmetabolicormorphogeneticactivityoftheepithelium,butiscausedbysomeepithelialproduct,probablyaprotein.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
