Synthesis of developmentally regulated proteins in Dictyostelium discoideum which are dependent on continued cell-cell interaction☆

[摘要] InthepreviouspaperweshowedthatthemajorchangesinthepatternofproteinsynthesisduringdifferentiationofDictyosteliumdiscoideumoccurduringthe4-hrperiodwhenthecellsareformingtight,visibleaggregates.Duringthistime,synthesisof10discretepolypeptidesmadebypreaggregationcellsceasesorisreducedconsiderably,andsynthesisof40newproteinsisinduced.InductionorcessationofsynthesisoftheseproteinswasparallelledbytheappearanceordisappearanceofthecorrespondingmessengerRNAs.Inthispaperweshowthatmanyofthesechangesareinducedbycontinuedcell-cellcontact.Noneoftheseoccursinaggregation-competentcellskeptinsuspensionculture,butchangesdotakeplacewhensuchcellsareallowedtoformtightaggregates.Disaggregationofcellscausescessationofsynthesisof“aggregation-stage”proteinsandreinductionofsynthesisofpolypeptidescharacteristicofpreaggregationcells.

[学科分类] 生物科学（综合）