Teratocarcinoma × friend erythroleukemia cell hybrids resemble their pluripotent embryonal carcinoma parent☆

[摘要] FiveindependentclonesofsomaticcellhybridshavebeenproducedbyfusingFBUFrienderythroblasticleukemiacellswithcellsofthepluripotentteratocarcinoma-derivedembryonalcarcinomalinePCC4azal.AllfivelinescloselyresembletheirPCC4azalparent.Theylooklikeembryonalcarcinomacellsbyphasecontrastandelectronmicroscopy,havehighlevelsofalkalinephosphatasebutlowlevelsofacetylcholinesterase,and,likePCC4azal,expressbothLDH-AandLDH-B.Tumorsproducedfromhybridlinesoftencontainlargeamountsofdifferentiatedtissue,includingrepresentativesofallthreeoftheclassicalgermlayers.Theseresultssuggestthatthegenomeofapluripotentmammaliancell,farfrombeingunconditionallysusceptibletowhateversignalsdifferentiatedcellsemploytomaintaintheirstablephenotype,mayitselfbeableto“reset”thegenomeofthedifferentiatedcell.

[学科分类] 生物科学（综合）