Flow of informational RNA from cytoplasmic poly(A)-containing particles to polyribosomes in Artemia salina cysts at early stages of development
[摘要] WhenArtemiasalinacystsareincubatedat28°Cinartificialseawater,thepoolofcytoplasmicmRNAasmeasuredbythe[3H]poly(U)hybridizationtechniqueisdepleted,andaparallelincreaseinthepolysomeboundmRNAisobserved.TheshiftofinformationalRNAfromsubribosomalparticlestopolyribosomesoccurswithoutasignificantincreaseintheamountofpoly(A)-containingRNAperembryo,atleastduringthefirsthoursofdevelopment.InordertoascertainwhetherresumptionofdevelopmentisduemainlytoutilizationofpreformedmRNA,thepolypeptidessynthesizedinawheatgermlysateunderthedirectionofcystcytoplasmicmRNAwerecomparedwiththoseobtainedusingpolysomalmRNAfromdevelopedembryos(1and4hr).Thecomplexpatternofproteinssynthesizedinvitrowasresolvedinto70–80radioactivespotsbytwo-dimensionalgelelectrophoresisandwasshowntobealmostidenticalinunincubatedandincubatedcysts,exceptforafewnovelspots.OurresultsdonotexcludethatearlytranscriptionofafewmRNAspeciescouldplayaroleintheactivationofmetabolicprocesseswhichoccurwhendevelopmentisresumed.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
