Absolute rates of protein synthesis in sea urchins with specific activity measurements of radioactive leucine and leucyl-tRNA☆
[摘要] Atechniqueforisolatingleucyl-tRNAanddeterminingitsspecificradioactivityisdescribed.Themethodisappliedtoananalysisoftheratesofproteinsynthesisinunfertilizedseaurchineggsandgastrulae.Comparisonofspecificactivitiesofleucineandleucyl-tRNAsuggeststhat,inthegastrulaunderourexperimentalconditions,compartmentationofintracellularleucineisrelativelyminor.Theproteinsyntheticrateinthegastrulastageembryois113timesgreaterthanthatintheunfertilizedegg.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
