Characterization of RNA synthesis in cultured lampbrush-stage Rana pipiens oocytes☆☆☆
[摘要] Intheprecedingpaper(RogersandBrowder,1976,Develop.Biol.55,135–147),wereportedthatbeginninglampbrush-stageoocytesassumemorphologicalcharacteristicssimilartomaximumlampbrush-stageoocytesafter24hrofculture.Thesechangesresultfromanapparentinvitroaccelerationofthelampbrushphase.Inthispaper,wecorrelatethemorphologyoftheculturedoocyteswithRNAsynthesis.Thechromosomesofculturedoocytesareactiveintranscription,asindicatedbyheavilylabeledlampbrush-chromosomeautoradiographs.ThenatureoftheRNAsynthesizedbythelampbrushchromosomeswasanalyzedbyanewlydevelopedtechniqueofdifferentialnucleasetreatmentof[3H]adenosine-labeledoocytesectionsandautoradiography.ResultsindicatethatlabelwasincorporatedintopolyadenylatedRNA,whichwasidentifiedbyitspartialresistancetoribonucleasesAandT1andbyitssusceptibilitytoribonucleaseT2.ThepolyadenylatedRNAisputativeinformationalRNAanditssynthesisindicatesthatthelampbrushchromosomesofculturedoocyteswereactiveinheterogeneousRNAsynthesis.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
