The polyadenylated RNA of zoospores and growth phase cells of the aquatic fungus, Blastocladiella☆

[摘要] Thestoredpoly(A)+RNAfromzoosporesoftheaquaticfungusBlastocladiellaemersoniirepresents2.5%ofthetotalRNAandhasamodelMWof425,000daltonsandanaveragepoly(A)isostichof32bases.Thepoly(A)+RNAalsorepresents2.5%ofthetotalRNAfromearlygrowthphasecellsandhasamodalMWof360,000daltonsandanaveragepoly(A)isostichof38bases.Thepoly(A)+RNAfromsporesand2-hrplantscontainsastructureresistanttoRNasesT1,T2,andA,whichcanbelabeledwith32PO4andwhichwillbindtoDBAE-cellulose.Thesecharacteristicsstronglysuggestthatboththezoosporepoly(A)+RNAandthe2-hrcellpoly(A)+RNAarecappedatthe5′end;and,hence,itisunlikelythatcappingisinvolvedinthecontrolofproteinsynthesisduringgermination.Approximately80%ofthepoly(A)+RNAofthesporeislocatedinthemembrane-enclosedribosomalnuclearcap,andmorethan90%ofthepoly(A)+RNAwithinthecapisfoundinthe80Smonoribosomeandheavierfractions.Synthesisofnewpoly(A)+RNAoccursveryearlyduringzoosporegermination,andthelabeledpoly(A)+RNArapidlyentersthenewlyorganizedpolysomes.Thelabelingdataforearlygerminationalsosuggestthatcytoplasmicpolyadenylationoccurs.

[学科分类] 生物科学（综合）