Hormonal regulation of epidermis-specific protein and messenger RNA synthesis in amphibian metamorphosis
[摘要] Experimentsusingamonospecificantibodydirectedagainstonetypeofepidermis-specifickeratinfromadultskinoftheamphibianXenopuslaevishavedemonstratedthatpolysomessynthesizingthisproteinfirstappearwithinlarvalskinduringnaturalmetamorphosis.Furtherexperimentsdemonstratedthatthesynthesisofkeratinwithinlarvalskincouldbeinducedprecociouslybythethyroidhormone,3,3′,5-triiodo-l-thyronine,bothinvivoandwhentheisolatedlarvalskinisculturedinvitro.TheearliestdevelopmentalageresponsivetosuchhormoneinductionappearedtobeStage5052oflarvaldevelopment.Thisisabout20–24daysbeforekeratinwouldnormallymakeitsappearancewithintheskinduringnaturalmetamorphosis.HormonetreatmentoftadpolesatthisagewillalsocauseaprecociousincreaseintheamountofkeratinmessengerRNApresentwithinlarvalskin.Thishasbeendemonstrateddirectlybytheisolationofpoly(A)-containingmessengerRNAfromhormone-treatedlarvaeanditstranslationinawheatgermcell-freesystemtogiveimmunoprecipitablekeratin.Peptideanalysisoftheinvitrotranslationproductindicatesthatthehormone-inducedmRNAprobablycodesforaninitialproteinproductthatisslightlylargerthankeratinitself.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
