Changes in rate of RNA synthesis and ribosomal gene number during oogenesis of Drosophila melanogaster☆
[摘要] AnewmethodforseparatingDrosophilaeggchambersintodifferentdevelopmentalclasses(Jacobs-LorenaandCrippa,1977)madeitpossibletostudychangesintherateofribosomalRNA(rRNA),5SRNA,andtRNAsynthesisandthechangesinribosomalgenenumberduringoogenesis.SynthesisofRNAwasmeasuredby[3H]uridineincorporationinvivoandsubsequentanalysisonsucrosegradientsorgelelectrophoresis.Specificradioactivityofnucleotidepoolshasalsobeendetermined.RibosomalgenenumberhasbeenmeasuredbyhybridizationofeggchamberDNAtorRNAofhighspecificradioactivity.OurfindingsledustoconcludethatinDrosophilamelanogaster:(i)rRNA,5SRNA,andtRNAaresynthesizedinallstagesofoogenesis.(ii)Ineverystage,rRNAisthemainRNAspeciessynthesized.(iii)TherateofrRNA,5SRNA,andtRNAsynthesisincreasesgreatlyduringoogenesisandisparalleledbyasimilarincreaseinribosomalgenenumberresultingfromthepolyploidizationofthenursecellnuclei.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
