Appearance of cell surface auto- and isoantigens during spermatogenesis in the rabbit
[摘要] Theappearanceofspermatogeniccellsurfaceauto-andisoantigenscanbepreciselydeterminedbyutilizingtechniquesthatseparatespermatogeniccells.Usingcytotoxic(complementdependent)auto-andiso-rabbitantirabbitwholesemensera,specificspermatogenicauto-andisoantigenswerefirstdetectedfollowingthematurationofspermatogoniaintoprimarypachytenespermatocytes.Theantiseraemployedwerecytotoxic(complementdependent)forpachytenediploteneandprimaryspermatocytesandspermatidsbutnotfortypeA,intermediate,ortypeBspermatogonia.Furthermore,Sertolicells,endothelialcells,Leydigcells,anderythrocyteswerenotlysedbytheantisera.Theseobservationssupporttheconceptofablood-testisbarrier.Onlyaftermigrationofspermatogoniatotheluminalsideofthebarriercanautoantigenicmoleculesbesynthesizedand/orinsertedintotheplasmamembraneofspermatogeniccells.Thus,theappearanceofsurfaceautoantigensoffersamodelsystemtostudythesynthesisofspecificmoleculeswhichareinsertedintotheplasmamembraneataprecisetimeduringdevelopment.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
