Synthesis of ovarian acid phosphatase-1 in Drosophila melanogaster☆☆☆
[摘要] Thesynthesisofacidphosphatase-1[MacIntyre,R.J.(1966a).ThegeneticsofanacidphosphataseinDrosophilamelanogasterandDrosophilasimulans.Genetics53,461–474]inthedevelopingovaryinDrosophilamelanogasterwasstudiedbiochemically,immunologically,andhistochemicallyattheultrastructurallevel.Theimpetusforthisstudywastheobservationthatacidphosphatase-1exhibitsamaternaleffectinearlyembryogenesis.Assayswhichmeasuretheamountofcross-reactingmaterial(CRM)againstantibodiesmadetoacidphosphatase-1stronglysuggestthatthisenzymeissynthesizeddenovointheovary,for,duringaspecifiedtime,therateofincreaseinCRMparallelstherateofincreaseinenzymeactivity.UsingtheleadphosphatemethodofGomoritolocalizeacidphosphatase-1histochemically,itwasdeterminedthatbothnursecellsandfolliclecellscontributetheenzymetothedevelopingoocyte;nursecellsapparentlytransporttheenzymeinlargeaggregatescontaininglysosomesthroughringcanalstotheoocyte.Smallpinocytoticvesiclescontainingacidphosphatase-1formintheoocyteatthefolliclecell/oocyteinterfaceduringthetimeofvitellinemembraneformation.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
