Induction of multiple proteases during the early larval development of Artemia salina☆

[摘要] ExtractsfromdormantanddevelopingembryosofArtemiasalinacontainalmostundetectablelevelsofproteaseactivity.However,afterhatchingofthenauplii,thereisahighincreaseintheactivityoftheextractsondifferentproteolyticsubstrates.Theactivitiescorrespondtofourdifferentenzymes,A,B,C,andD,thathaveasequentialtimingofinductionduringtheearlylarvaldevelopment.TheenzymesshowadifferentelutionpatternafterchromatographyonDEAE-celluloseandadifferentsensitivitytoproteaseinhibitors.EnzymeAisstronglyinhibitedbyphenylmethylsulfonylfluoride,whiletheotherenzymesaremostlyinsensitive.EnzymesA,B,andCaresensitiveatlowconcentrationsofthesoybeantrypsininhibitor.Thetemporalinductionoftheseenzymesisanexampleoftheexpression,atthebiochemicallevel,ofthedevelopmentalprogramofArtemiasalina.

[学科分类] 生物科学（综合）