Analysis of glial cell differentiation in peripheral nervous tissue: I. S100 accumulation in quail embryo spinal ganglion cultures☆
[摘要] LevelsofS100,aproteincharacteristicofnervesupportivetissue,undergotwodistincttransitionsduringthedevelopmentofavianembryonicspinalganglia.Radioimmunoassayshowsthatbothtransitionsalsooccurinculturedspinalganglia.However,homogeneousculturesofsupportivecellprecursorsfrom5-dayembryonicspinalgangliafailtoundergotheseconddevelopmentaltransitionmarkedbylargeincreasesofS100.Immunohistochemistry,utilizingperoxidase-anti-peroxidaseprocedures,revealsthathomogeneoussupportivecellculturesstainlightlyanduniformlyforS100whereaswholeganglionculturescontainsomeintenselystainedcells,whichareonlyassociatedwithneuroncellbodiesorfibers.Neurons,therefore,mayberequiredtoinducehighS100levelsindifferentiatingsupportivecells.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
