Cell surface binding sites for peanut agglutinin in the differentiating eye disc of Drosophila☆
[摘要] Thedistributionofpeanutagglutinin(PNA)bindingsitesinimaginaldiscsisdescribedusingfluorescenceandelectronmicroscopy.PNAbindspreferentiallytothephotoreceptorcellprecursorsineyediscsresultinginarectilineararrayoffluorescentspotsthatreflectsthatlattice-likearrangementofthepresumptiveommatidia.Thelectinbindstotheapicalsurfaceoffixeddisccellsandistakenupinpresumedendocytoticvesiclesinlivingdiscs.Photoreceptorprecursorscanbevisualizedwithfluoresceinisothyocyanate-PNAfromthetimetheyfirstformpreclustersinthemorphogeneticfurrowandthistechniqueisusedtodemonstrateatemperature-sensitivedefectinpreclusterformationinthemutantshibire.PNAislocalizedalongthesidesofmicrovilliofdisccells,ingeneral.ThepreferentialbindingofPNAtophotoreceptorprecursorsisrelatedinparttothehighdensityofapicalmicrovillionthesecells.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
