The formation of the pupal cuticle by Drosophila imaginal discs in vitro☆
[摘要] Mass-isolatedimaginaldiscsofDrosophilamelanogasterformachitin-containingpupalprocuticleInvitro.Optimalprocuticledepositionoccurswhenthediscsareincubatedfor4–6hrwith0.5–1.0μg/mlof20-hydroxyecdysoneandthenwithlessthan0.05μg/mlof20-hydroxyecdysone.Theformationofthechitin-containingprocuticleisdemonstratedusingthreeindependentassays:withfluorescene-conjugatedcuticleproteinsthatbindtochitin;byelectronmicroscopy;byincorporationof[3H]glucosamineintoachitinfraction.Synthesisanddepositionofpupalcuticleproteinsarealsodemonstrated.Incorporationof[3H]glucosamineintochitinissensitivetoinhibitorsofprotein,RNAandchitinsynthesis,buthaslittlesensitivitytoinhibitorsofDNAsynthesis,anddolichol-dependentglycosylation.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
