Evidence for the guidance of pronephric duct migration by a craniocaudally traveling adhesion gradient☆☆☆
[摘要] TheseexperimentswereundertakentoidentifythegeneralnatureofthemechanismthatguidesthemigrationoftheAmbystomapronephricductalongtheventraledgeofthesomitefilefromitsanteriororigintothecloaca.Usingscanningelectronmicroscopyinconjunctionwithmicrosurgery,wehavesoughttodistinguishamongsuchpossibilitiesaschemotaxis,contactguidance,andgradientsofadhesivenesstothesubstratum.Apronephricductprimordiumtransplantedtotheflankofahostventraltotheprimaryductmigratesdorsocaudallyacrosstheflanktofusewiththeprimaryduct.Removalofpotentialsourcesofdistantattractiondoesnotalterthisbehavior,nordomigratingsecondaryductsfollowanyvisiblestructures.Avarietyoftransplantationexperimentsrevealthattheguidanceinformationisnotonlyorientedbutalsodirectionallypolarizedandtravelscaudadasawave.Theseresultsmilitateagainstchemotaxisandcontactguidanceasguidinginfluencesandindicatethatthecellsofthepronephricducttiparedirectedintheirmigrationbylocalinformationwhichpassescaudadovertheduct'smesodermalsubstratumasawaveinregisterwiththeadvancingwaveofsomitesegmentation.Weproposethatthisduct-guidinginformationmaybeatravelinggradientofflankmesodermcelladhesiveness.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
