Developmental lesions in the Agametic mutant of Drosophila melanogaster
[摘要] Agametic,amaternal-effectmutation,causestheabsenceofgermcellsinapproximately40%ofthegonadsoffliesderivedfromhomozygousfemales.Thenatureofthedeficiencyintheeggsproducedbytheseflieswasexamined.Ultrastructuralabnormalitieswereseeninthepolargranulesofsomeeggsshortlyafterfertilization.Althoughanormalnumberofpolecellsform,someareabnormalwithdegeneratingpolargranulesandnuclearbodiesandtheycontainmyeloidbodies.Thepolecellsreachthegonadsandat14hrofdevelopmentallthegonadscontaingermcells.However,in40%ofthegonadsthegermcellsbecomenecroticanddisappear.Thus,thesourceofagameticgonadsintheadultisembryonicdeathofpolecellsinsomegonads.Totestwhetherthisgonadaldeathisanautonomousdeficiencyofthemutantpolecells,mosaicpolecellpopulationswereproducedbyreciprocalpolecelltransplantation.Inbothtypesoftransplants,themutantpolecellsdiedautonomously.Ineightinstancesgonadscontainingonlydonorpolecellswereobtained.Sincemutantpolecellsdiewhenwild-typepolecellsnormallybegindividing,wesuggestthatthelesionaffectstheabilityofthesemutantpolecellstoreenterthecellcycle.
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[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
