Protein synthesis during maturation promoting factor- and progesterone-induced maturation in Xenopus oocytes☆
[摘要] ThequantitativeandqualitativeaspectsofproteinsynthesisduringXenopusoocytematurationwereinvestigated.Progesteroneortheinjectionofmaturationpromotingfactor(MPF)bothincreasedproteinsynthesisapproximatelytwofoldrelativetocontrols.However,MPFinducedaprecociousincreaserelativetothetimeofgerminalvesiclebreakdowncomparedtothatobservedinresponsetoprogesterone.Examinationoftwo-dimensionalgelsshowedminimalchangesinthetypesofpolypeptidessynthesizedfromcontrolandprogesterone-andMPF-maturedoocytes.RathertherewasanoverallincreaseinthesynthesisofindividualpolypeptidesduringprogesteroneandMPF-inducedmaturation.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学(综合)
[关键词] [时效性]