A switch for transdifferentiation in culture: Effects of glucose on cell determination in chick embryo neuroretinal cultures☆
[摘要] Chickembryoneuroretinalcellsaccumulatelens-specificδ-crystallinwhenculturedinEagle'sminimalessentialmediumcontaining5%foetalcalfserumand5%horseserum(FH),butfailtodosoifsupplementaryglucoseispresent(FHG).Culturegrowthratesaresimilarinthetwomedia,butcholineacetyltransferaseactivityismaintainedforlongerinFHGmedium.BytransferringculturesfromFHGintoFHmediumandviceversa,weshowthatpotentiallensprecursorcellssurviveforatleast21daysinFHGmedium,whilesomeretinalcellsbecomeirreversiblycommittedtocrystallinproductionafter18daysinFHmedium.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
