Sorting behavior among cells from the 14-day embryonic chick neural retina☆
[摘要] Wehavedevelopedaprocedurefortheisolationoffourdifferentviablecellpopulationsfromthe14-dayembryonicchickneuralretina.PopulationIconsistsprimarily(>80%)ofcellswhichappeartobeganglioncells.PopulationIVconsistsprimarily(>90%)ofsmallcellswhichappeartooriginatefromthebipolarlayer.PopulationsIIandIIIaremixturesofseveralcelltypes.Cellsofeachpopulationwerelabeledwitheitherrhodamineisothiocyanateorfluoresceinisothiocynate,andmixedtogetherunderconditionswhichpromotecellaggregation.Thelabelallowedustofollowthefateofthedifferentcellpopulationsinmixtures.Inallheterotypicmixtures,distinctcellsortingwasobserved.Controlsindicatedthatthelabelingproceduredidnotalterthebehaviorofthecellsinvitro,andthathomotypicmixturesdidnotsortout.Theseresultssuggestthatanintercellularrecognitionsystemexistswhichpermitsthecellsinthepopulationstodiscriminateamongeachother.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
