Inhibition of myoblast fusion by lysosomotropic amines☆
[摘要] Fusionofculturedchickembryomyoblastswasinhibitedbytreatmentwithseverallysosomotropicamines.Theconcentrationsrequiredforhalf-maximalinhibitionoffusionwereapproximately2μMforchloroquine,30μMfortributylamine,3.2mMforammoniumchloride,and3.3mMformethylamine.Alltheaminesinhibitedfusionappreciablyatconcentrationslowerthanthosethatreducedcelldensity.Boththerateandextentoffusionwereaffectedbytheamines,whichhadtobepresentforabout20hrbeforetheusualonsetoffusion.Inhibitionoffusionwasreversiblebytransferofinhibitedcellstofreshmedium.Theaminesdidnotcauseaccumulationofanondialyzableinhibitorintheculturemedium.Levelsofcreatinekinaseincreasedbyeight-foldormorebetween18and65hrinculturestreatedwithtributylamineorchloroquine,althoughthisincreasewasnotaspronouncedasincontrolcultures.Theincreasedcreatinekinaseactivityinamine-treatedcultureswasduemainlytotheBBandMBisozymes,withrelativelylittleincreaseintheMMisozyme.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
