Survival of isolated, fully grown mouse ovarian oocytes is strictly dependent on external Ca2+☆
[摘要] ThepresenceofCa2+isessentialforsurvivalincultureoffullygrownoocytesisolatedfrommouseovariesbutnotforsurvivalofsmall,meioticallyincompetentoocytes,metaphaseIIoocytes,andearlyembryos.Ninetypercentoffullygrownovarianoocytesdiewithin2hrwhenculturedincalcium-freemedium(CFM).CFMdeathdoesnotoccurifothercations(1mMLa3+or10mMSr2+,butnot12mMMg2+nor1mMD-600)replaceCa2+inthemedium.SensitivitytoCFMisprogressivelyacquiredbytheoocyteduringthegrowthphase,inparallelwiththeacquisitionofmeioticcompetence,andislostafter2hrofcultureinthepresenceofatleast0.5mMCa2+.ThelossofsensitivitytoCFMduringinvitrocultureisnotrelatedtotheconcomitantspontaneousresumptionofmeiosis,sincetheoocytebecomesresistanttoCFMevenifgerminalvesiclebreakdownispreventedbytheadditionofdibutyrylcAMPtotheculturemedium.Somehypothesesareputforwardtoexplainthepeculiarandtransienthighcalciumrequirementsoffullygrownoocytes.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
