Messenger RNA encoding basic chromosomal proteins of mouse testis☆
[摘要] Considerableknowledgehasaccumulatedconcerninghistonesandotherbasicchromosomalproteinswhichappearinmammalianmalegermcellsduringspermatogenesis.Thebestcharacterizedoftheseproteinsaretestisprotein(TP)andprotamine.Botharesmallandhighlybasic,TPbeingpresentinearlyspermatidswhiletwomolecularspeciesofmouseprotaminemaybedetectedinspermatidsinthelaterstagesofdifferentiationandinsperm.TheaminoacidsequenceofTPfromratandofasinglespeciesofprotaminefrombullhavebeenreported.Incontrasttothisdetailedinformationrelatedtoproteinstructure,relativelylittleisknownofthemechanismsinvolvedinsynthesisoftheproteinsorofthecontrolswhichregulatetheirsynthesisduringspermatogenesis.Inthestudyreportedhere,messengerRNA(mRNA)frommousetestiswasexaminedforitsabilitytoactasatemplateforinvitrosynthesisofTPandprotamine.ThreepolyacrylamidegelelectrophoresissystemsweredevelopedtodistinguishTPandthetwospeciesofmouseprotamineamonginvitrotranslationproductssynthesizedbyacell-freewheatgermextract.A6SRNAencodingTPwasidentifiedbytranslationofsucrosegradientfractions.RNAencodingTPwasalsofoundamongthetestisRNAswhichwereboundtooligo(dT)-celluloseduringaffinitychromatography.ThesephysicalpropertiesandapparentabundanceofthisTPmRNAsuggestthatitmaybeausefulprobeforfurtherstudiesofRNAmetabolismandgeneregulation.Innocasewasaninvitrotranslationproductcomigratingwiththetwoprotaminespeciesobserved,suggestingthepossibilitythatprotaminemaybesynthesizedasaprecursorandlaterprocessedtothespecieswhicharefoundinsperm.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
