Inhibition of branching morphogenesis and alteration of glycosaminoglycan biosynthesis in salivary glands treated with β-d-xyloside☆

[摘要] Theroleofglycosaminoglycans(GAGs)inthebranchingmorphogenesisofembryonicmousesalivaryglandswasinvestigatedbyculturingtheglandsinthepresenceofxylosederivativeswhichstimulatesynthesisofthexyloselinkedclassesofGAGs.Branchingmorphogenesisisinhibitedseverely,butreversibly,by0.5–1.0mMÏ€-nitrophenyl-β-d-xylopyranosideandtheinhibitioncorrelateswithastimulationofincorporationof[3H]glucosamine(1.8-fold)and[35S]sulfate(almost3-fold)intoGAGs.Theeffectofβ-xylosideonaccumulationofnewlysynthesizedGAGalsooccursinthepresenceoftheproteinsynthesisinhibitorcycloheximide,suggestingthattheproductionoffreeGAGchainsratherthanproteoglycan-associatedGAGsisbeingstimulated.Thexylosideeffectsapparentlydonotresultfromgeneralcytotoxicityofthederivatives,sincesimilarconcentrationsoftheα-anomerdonotaltersalivarybranchingorGAGsynthesis,therudimentsresumemorphogenesiswhenreturnedtocontrolmedium,andtheeffectonGAGsynthesisisstimulatoryratherthaninhibitory.ThestudysuggeststhatGAGbiosynthesisplaysanimportantroleinsalivarydevelopment,andthatxylosidesprovideusefulprobesforcharacterizingthemoleculareventscontrollingbranchingmorphogenesis.

[学科分类] 生物科学（综合）