Some somatic sequences are absent or exceedingly rare in Xenopus oocyte RNA☆

[摘要] AvarietyofXenopuslaeviscDNAclonesderivedfromsomaticcellRNAswerehybridizedtooocytepA+RNAseparatedonNortherngels.WewereunabletodetectoocytepA+sequencescomplementarytothreeundefinedtadpolecDNAclones.Withoneoftheseclones,acomplexpatternofbandsappearsduringembryogenesis.Withtheothertwoclones,asinglebandappears.TwoadditionaltadpolecloneshybridizetobothoocyteandtadpoleRNA,butyieldamorecomplexRNApatternfromembryosthanfromoocytes.OneoftheseadditionaltadpolecloneshascomplementaritytoactinDNA,suggestingthattheadditionalRNAbandwhichappearsduringembryogenesisisα-actinmRNA(E.A.Sturgess,J.E.M.Ballantine,H.R.Woodland,P.R.Mohun,C.D.Lane,andG.J.Dimitriadis,1980,J.Embryol.Exp.Morphol.58,303–320).WehavealsofailedtodetecthybridizationtooocytepA+RNAwithonevitellogeninandthreeadultglobincDNAclones.ReconstructionexperimentswithpurifiedglobinmRNAfromanemicadultbloodcellssetthelowerlevelofsensitivityforglobinmRNAatonepartin106.ThedatasuggestthatsomeXenopusmRNAsequencesareabsentorveryrareintheoocytepA+RNApopulation.

[学科分类] 生物科学（综合）