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Protein synthesis and accumulation in Drosophila melanogaster imaginal discs: Identification of a protein with a nonrandom spatial distribution☆
[摘要] ProteinsfromDrosophilaimaginaldiscsanddiscfragmentswereanalyzedontwo-dimensionalelectrophoreticgelsfollowinglabelinginvitrowith[35S]methionine.Theproteinsyntheticpatterninautoradiogramsisverycomplexandparallelsthepatternofproteinaccumulationvisualizedinsilver-stainedgels.Wefindnoreproduciblequalitativedifferencesintheproteinssynthesizedoraccumulatedbydifferentdisctypes.Additionally,analysisoftheproteinssynthesizedbydifferentfragmentsofwingandhalterediscshasresultedintheidentificationofapolypeptidewhichissynthesizedpreferentiallyinhomologousregionsofthesetwoimaginaldiscs.Scanningdensitometryofourautoradiogramscorroboratesthesefindings.Thisprotein,therefore,hassomeofthepropertiesonewouldpredictforamoleculeinvolvedintheimaginaldiscpositionalinformationsystem.
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[效力级别]  [学科分类] 生物科学(综合)
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