Transient localizations of messenger RNA in Xenopus laevis oocytes☆

[摘要] Thedistributionofpoly(A)-containingRNA[poly(A)+RNA]wasexaminedinsectionsofoocytes,matureeggs,andearlyembryosofXenopuslaevisbyinsituhybridizationwith[3H]poly(U).Poly(A)+RNAwasuniformlydistributedinthecytoplasmofprevitellogenicoocytes.Incontrast,distinctcytoplasmicregionsexhibitinghighconcentrationsofpoly(A)+RNAweredetectedinvitellogenicandfull-grownoocytes.Theseregionsincludeaperinucleararealocatedbetweenthegerminalvesicle(GV)andthecortexofstage3oocytes,acorticalareainstage4oocytes,avegetalsubcorticalareaandasubnuclearzoneinstage5oocytes,andavegetalsubcorticalareainstage6oocytes.Noselectiveaccumulationsofpoly(A)+RNAwereobservedinthemitochondrialmassofprevitellogenicoocytesorintheGVatanytimeduringoogenesis.Thepoly(A)+RNAlocalizationsintheperipheralregionsoffull-grownoocytesdisappearedduringthefirstmaturationdivisionleadingtoauniformpoly(A)+RNAdistributioninthematureegganddidnotreappearduringearlyembryogenesis.Theresultssuggestthattransientlocalizationsofpoly(A)+RNAarepresentintheoocytecytoplasmduringoogenesisofXenopuslaevis.

[学科分类] 生物科学（综合）