Channel open time of acetylcholine receptors on Xenopus muscle cells in dissociated cell culture☆
[摘要] Thekineticsofacetylcholine(ACh)receptorchannelsonculturedmyotomalmusclecellsfromXenopusembryoswerestudiedbyanalyzingfocallyrecordedmembranecurrents.Themeanopentimeforreceptorchannelsonembryonicmusclecellsgrownindissociatedcellculturesshowedatime-dependentdecreasesimilartothatseeninvivo.ThechangesinpowerdensityspectraareconsistentwiththehypothesisthatthedecreaseresultsfromtheappearanceofaclassofAChreceptorwithashortmeanchannelopentime(0.7msec)andadecreaseintheproportionofreceptorswithalongmeanchannelopentime(3msec).TheadditionofdissociatedneuraltubecellstomusclecellculturesresultedinanunexpectedincreaseinmeanchannelopentimeforAChreceptorsinbothsynapticandnonsynapticregions.ThesestudiesdemonstratethatAChreceptorfunctionmaybealteredinculturedmusclecells.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
