Effects of cholera toxin and actinomycin on synthesis of [35S]methionine-labeled proteins during progesterone-induced maturation of Xenopus laevis oocytes☆
[摘要] Theincorporationof[35S]methionineintopolypeptidesduringprogesterone-inducedmeioticmaturationofXenopuslaevisoocyteswasstudiedbytwo-dimensionalpolyacrylamidegelelectrophoresis.Fivemodificationswereconsistentlyobserved:twopolypeptidesofanapproximatemolecularweightof150KdaltonsandpI5werenewproteins,tworepresentincreasedincorporationandonewasdecreasedincorporation.Choleratoxininhibitedtheappearanceofthemodificationsinducedbyprogesterone.Actinomycinandenucleationdidnotsignificantlyalterthemodifications.Thesedataindicatethatagoodcorrelationexistsbetweenthemodificationsinproteinsynthesisinducedbyprogesteroneandtheresumptionofmeioticcelldivision.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
