Developmental patterns of cytoplasmic transcript prevalence in sea urchin embryos☆
[摘要] Severalhundredtwo-cellstage,gastrulastage,andpluteusstageseaurchinembryocDNAcloneswerescreenedwith[32P]cDNAtranscribedfromadevelopmentalseriesofcytoplasmicandpolysomalpoly(A)RNAs.TranscriptprevalencewasestimatedbyreferencetothereactionofaseriesofstandardclonescomplementarytoRNAsofknownabundance.Wedescribethedominantpatternofprevalencechangeduringdevelopmentforabundantsequences,i.e.,thosepresentintherange5×104–106molecules/embryo.Thesetranscriptsaremostoftenaboutequallyabundantineggandpluteusstageembryos,andareseveralfoldlessprevalentatgastrulastage.Lessthan10%ofthesequencesdisplayingthispatternundergo>10-foldchangesinprevalenceduringdevelopment.Asearchforlaterembryosequencesnotrepresenteddetectablyineggorcleavagestageembryopoly(A)RNAyieldedanumberofexamples.ThesewereconfirmedbytheRNAgelblotmethod.Sequencesregulatedinthismannerallbelongedtolowerabundanceclasses,andarepresentatabout40copies/cellorlessatthegastrulastage.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
