Transcription of Xenopus borealis rRNA genes in nuclei of Xenopus laevis oocytes☆
[摘要] RecombinantplasmidsthatcontainedasinglerRNAgeneandspacerfromXenopusborealiswereinjectedintothenucleiofXenopuslaevisoocytes.Afterspreadingthecontentsofinjectednucleiforelectronmicroscopy,wefoundtranscriptionunitsindicativeoftheaccuratetranscriptionoftheborealisgene.BoththenumberofRNApolymerasemoleculespertranscriptionunitandthetotalnumberofactivegenespernucleusweresimilartothosefoundforinjectedlaevisgenes.WealsoinjectedlaevisoocyteswithaplasmidthatcontainedbothashortenedlaevisrRNAgeneandafull-lengthborealisrRNAgene.Visualizationoftranscriptiononthisplasmidshowedthatusuallyeitherashortoralongtranscriptionunitwaspresentonanyonemolecule.Themorphologiesoftheshortandlongtranscriptionunitswereconsistentwiththeirrepresentingtranscriptionofthelaevisandborealisgenes,respectively.Bothtypesoftranscriptionunitwerepresentinroughlyequalnumbers.SincethelaevisoocytetranscribedinjectedborealisrRNAgenesasefficientlyasittranscribedinjectedlaevisrRNAgenes,itstandsinmarkedcontrasttothesituationinembryosofinterspecifichybridsbetweenlaevisandborealiswheretranscriptionoccursonlyfromthelaevisrRNAgenes.PossibleexplanationsforthedifferenceintranscriptionalbehavioroftheborealisrRNAgeneinhybridembryosandlaevisoocytesarediscussed.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
