Analysis of glial cell differentiation in peripheral nervous tissue: II. Neurons promote S100 synthesis by purified glial precursor cell populations☆
[摘要] IsolatedsensoryneuronsinvitrodonotcontainorsynthesizeS100,whereasglialcellprecursorpopulationsdo.Theseprecursorcells,whenisolatedfromothercelltypes,producelowlevelsofS100butneverundergothedevelopmentaltransitiontoproducehighlevelsofS100.Whenglialcellprecursorsarecombinedwithisolated,liveorparaformaldehyde-fixedsensoryneurons,theprecursorcellsdoundergothesecondtransition,andaccumulatehighlevelsofS100.Peroxidase-anti-peroxidaseimmunohistochemicalstainingforS100confirmspreviousconclusions(B.HoltonandJ.A.Weston,1982,Develop.Biol.89,64–71)thatonlythoseglialcellswhicharecloselyapposedtoneuronscontainaugmentedlevelsofS100.Thisstimulationappearstobespecifictoneuronal/glialinteractionssinceliveorfixedfibroblasts,whencoculturedwithglialprecursorcells,donotpromoteaccumulationofS100bytheglialcells.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
