Sea urchin hyalin: Appearance and function in development
[摘要] Hyalin,theextracellularmatrixproteinofseaurchinembryos,wasexaminedbyimmunofluorescence.Priortofertilizationhyalinwasconfinedtocorticalgranules.Atfertilizationhyalinwasreleasedfromsomeofthecorticalgranulesaspartofthevisiblefertilizationwave.Furtherhyalinaccumulationonthecellsurfaceoccurredinthesucceeding2–3minasadditionalcorticalgranuleswerereleased.Thehyalinfusedtoformacontinuousextraembryoniclayer.Regenerationstudiesshowedthatnewaccumulationsofhyalinwereinitiatedbyectodermcellsatearlygastrulation.Regenerationdidnotoccurpriortothisdevelopmentaltime,norwastherenewhyalinaccumulationonmesodermorendodermcells.Hyalinaccumulatedonlyontheapicalsurfaceofectodermcells.Inbindingstudies,cellsofallthreegermlayershadanaffinityforhyalinpriortogastrulation.Theaffinitywasretainedbyectodermandendodermbutwaslostbyprimarymesenchymecellsatthebeginningofgastrulation.Thelossinaffinitywasspecificforhyalinandoccurredatthetimewhenprimarymesenchymecellsnormallyleavetheblastodermtoinvadetheblastocoel.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
