Transdifferentiation and regeneration in vitro☆
[摘要] StriatedmuscletissueandendodermcanbeisolatedfromtheanthomedusaPodocorynecarnea.Theisolatesareuncontaminatedbyothercelltypesandcanbecultivatedinartificialseawaterformonthswithoutundergoingautonomousregeneration.However,iftheendodermiscombinedwithcollagenase-treatedstriatedmuscle,aregenerationprocessisinitiatedwhichleadstotheformationofthesexualandfeedingorgan(manubrium)ofthemedusa.Theoriginalendodermandstriatedmusclearereplacedintheregeneratebyatleastsevennewcelltypes,includinggametes.Labelingexperimentswith[3H]thymidineandexperimentsinwhichmitosisisinhibitedineitherthestriatedmuscleortheendodermwithmitomycinCdemonstratethatthestriatedmuscleisabletotransdifferentiateintoallthecelltypesfoundintheregenerate.Withthepossibleexceptionofectodermalsmoothmusclethisstatementisalsovalidfortheendoderm.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
