Accumulation of individual histone mRNAs during embryogenesis of the sea urchin Strongylocentrotus purpuratus☆
[摘要] WehavedeterminedthatthestockpileofmaternalhistonemRNAsforbothH2BandH3ineggsoftheseaurchinStrongylocentrotuspurpuratuscorrespondsto0.8–1millionmoleculesperegg.Thisvalueisasomewhatsmallerquantitythanhadpreviouslybeenestimated.Ontheotherhand,theincreasebroughtaboutbyembryonicmRNAsynthesisis10-to12-fold,ratherthan4-fold.FurtheranalysisofthetimecourseofaccumulationofthesemRNAssuggeststhathistonegeneexpressioninearlydevelopmentinvolvesacomplexinteractionbetweenactivationofstockpiledmaternalmessengers,fluctuatingratesofhistonegenetranscription,andalterationsofhistonemRNAstability.ThematernalpoolofH1mRNAisconsiderablysmaller(80,000molecules).Duringcleavage,itpartiallycatchesupandpeaksatabouthalfthevalueforH2BandH3.WealsoexaminedprevitellogenicoocytesforthepresenceofhistonemRNAsbythesamemethodsandwereunabletodetectany.ThusthestockpileofmaternalhistonemRNAsmustaccumulateaftertheprevitellogenicstageofoogenesis.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
