Development of cholinergic neurons of the rat retina
[摘要] Biochemicalandelectrophysiologicalfeaturesofcholinergicneuronsfromthedevelopingratretinawereanalyzedinanattempttoidentifyimportantperiodsinthematurationoftheseneurons.Acetylcholinesynthesiswasassayedinintact,isolatedretinas;cholineacetyltransferaseactivitywasmeasuredinhomogenates.Spontaneousandevokedacetylcholinereleaseandcertainaspectsofsynapseformationwereexaminedinaretina-musclecellculturesystem.Inthissystem,retinalcellsfromratsofdifferentagesweredissociatedwithtrypsinandaddedtopreviouslyculturedratstriatedmusclecellswhichservedaspostsynaptictargetsforcholinergicneurons.Theresultsindicatethattwodevelopmentalperiodscanbedescribed.Thefirststageoccursduringthefinalweekofgestationwellbeforemorphologicalsignsofsynapsesappear.Duringthisearlyperiod,cholinergicneuronsacquiretheabilitytosynthesizeandreleaseacetylcholineandtoformfunctionalsynapses.Thesedevelopingneuronsprogressivelylosetheirabilitytoformandtomaintainsynapseswithaninappropriatetarget.Asecondstageofmaturationbeginsattheendofthefirstpostnatalweek.Inthisphase,dramaticincreasesinacetylcholinesynthesis,cholineacetyltransferaseactivity,andhigh-affinitycholineuptakearecoupledtemporallywiththemorphologicaldifferentiationofsynapses.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
