Identification of vitelline membrane proteins in Drosophila melanogaster☆
[摘要] InDrosophilamelanogaster,proteinsinvolvedinvitellinemembraneproductionaresecretedbyovarianfolliclecellsduringstages9and10ofoogenesis.WehaveusedSDS-PAGEandtwo-dimensionalelectrophoresistoidentifysixmajorsizeclassesofradiolabeledcomponentsinpurifiedvitellinemembranepreparations.Analysesofinvivolabeledproteinsfromeggchambersofdifferentdevelopmentalstagesandstage10folliclecellsshowthatcomponentsoffiveofthesesizeclassesaresynthesizedbyfolliclecellsduringtheperiodofvitellinemembranedeposition.Immunologicalanalysisofeggshellantigensutilizingcomplexantiseraraisedtopurifiedeggshellfragmentshasconfirmedtheidentityofcomponentsofthreesizeclasses.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
