Changes in the pattern of protein phosphorylation during meiosis reinitiation in starfish oocytes
[摘要] EndogenousproteinphosphorylationinoocytesofMarthasteriasglacialiswasexaminedbyincubatinglivingoocyteswith[32P]phosphateandcorticalandendoplasmicfractionswith[γ-32P]ATP.Individualphosphorylatedproteinsweredetectedbyautoradiographyafterbidimensionalandmonodimensionalelectrophoresis,usingSDS-polyacrylamidegradientgelslabs.Anincreasedphosphorylationofseveralproteinspecieswasobservedasearlyas5minfollowinginvivohormonalstimulationby1-methyladenine.Nodephosphorylationnoranychangeinproteinstainingofthegelswasobserved.Invitrophosphorylationpatternswereconsistentwiththoseobservedinvivo.Theydidnotchangeuponinvitro1-methyladenineadditionandremainedunaffectedwhentheincubationswerecarriedoutinthepresenceofcAMPorbeefheartproteinkinaseinhibitor.Corticalphosphorylationwasinhibitedbycalciumions.Theresultssuggestthatthehormonepromotesalterationsintheavailabilityofphosphorylationsitesinsomeproteinsalreadypresentinthecontroloocyteswhich,aswellasthecorrespondingactivatedcAMP-independentproteinkinases,mayplayasignificantroleduringformationofthematurationpromotingfactor.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
